ASTM E2196-2007 用旋转盘式反应器量化随剪切连续流生长的铜绿假单胞菌生物膜的标准试验方法

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【英文标准名称】:StandardTestMethodforQuantificationofaPseudomonasaeruginosaBiofilmGrownwithShearandContinuousFlowUsingaRotatingDiskReactor
【原文标准名称】:用旋转盘式反应器量化随剪切连续流生长的铜绿假单胞菌生物膜的标准试验方法
【标准号】:ASTME2196-2007
【标准状态】:现行
【国别】:
【发布日期】:2007
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E35.15
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:连续的;圆盘;流动;微生物学;假单胞菌;量化;反应器;旋转;剪切
【英文主题词】:analysis;biofilm;coupon;growthreactor;Pseudomonasaeruginosa;reactor;sampling
【摘要】:Bacteriathatexistinabiofilmarephenotypicallydifferentfromsuspendedcellsofthesamegenotype.Thestudyofbiofilminthelaboratoryrequiresprotocolsthataccountforthisdifference.Laboratorybiofilmsareengineeredingrowthreactorsdesignedtoproduceaspecificbiofilmtype.Alteringsystemparameterswillcorrespondinglyresultinachangeinthebiofilm.Thepurposeofthismethodistodirectauserinthelaboratorystudyofbiofilmsbyclearlydefiningeachsystemparameter.Thismethodwillenableapersontogrow,sample,andanalyzealaboratorybiofilm.1.1ThistestmethodisusedforgrowingarepeatablePseudomonasaeruginosabiofilminacontinuouslystirredflowreactor.Inaddition,thetestmethoddescribeshowtosampleandanalyzebiofilmforviablecells.1.2Inthistestmethod,biofilmpopulationdensityisrecordedaslogcolonyformingunitspersurfacearea.1.3Basicmicrobiologytrainingisrequiredtoperformthistestmethod.Thisstandarddoesnotclaimtoaddressallofthesafetyproblemsassociatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetypracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:G92
【国际标准分类号】:07_100_01
【页数】:5P.;A4
【正文语种】: